Flying with a Dog from United States to Spain — Requirements

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Vaccinations

Rabies vaccination
Valid rabies vaccination required. Vaccine validity begins a minimum of 21 days after completing the primary vaccination protocol. The dog must be at least 12 weeks old at the time of vaccination.
Before 21 days entry Official source

Documents

EU Animal Health Certificate
An Animal Health Certificate (AHC) must be completed and issued by a government-accredited official veterinarian in the USA. Valid for 10 days from issue. Your dog must enter through a designated EU travellers' point of entry where documentary and identity checks are carried out.
Before 10 days entry Valid for 10 days Official source
Non-commercial movement declaration
A written declaration signed by the owner (or authorised person) confirming the movement is non-commercial — i.e. the pet is not being sold or transferred. Must accompany the health certificate.

Tests

Rabies antibody titer test (≥ 0.5 IU/ml)
Because the USA is not EU-listed, a rabies antibody blood test is required. The blood sample must be taken at least 30 days after the rabies vaccination. Testing must be done at an EU-approved laboratory. You must wait 3 months (90 days) after the blood sample is taken before entering the EU. Result must be ≥ 0.5 IU/ml.
Before 90 days entry Official source

Microchip

Microchip (ISO 11784/11785)
Your dog must be marked with a microchip to ISO standards. The microchip must be implanted before or on the same day as the first rabies vaccination.

Other Requirements

Tapeworm treatment (Echinococcus) — dogs only
Dogs must be treated for Echinococcus tapeworm by a vet between 24 and 120 hours (1–5 days) before entry. The treatment must be documented in the animal's health certificate.
Before 1 day entry Official source

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